Abstract
Abstract A simple test-tube semi-micro method for the determination of total phosphorus in water is described. Samples are evaporated with perchloric acid at 115 °C overnight, digested at 170 °C for 40 minutes, diluted, hydrolyzed at 100 °C for 1 hour in order to decompose pyrophosphate and the liberated orthophosphate is determined without further neutralization and transfer steps by the phosphomolybdate blue method of Murphy and Riley (1962). The method can be applied directly to samples with phosphorus concentrations ranging from 4 to 1000 μg.1−1. Comparability with the classic perchloric acid and sulphuric acid—persulphate digestion methods has been demonstrated using a wide range of fresh waters.
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