Abstract
Semiautomated fluorometric and colorimetric methods for the determination of reserpine in single tablets are described for 0.25- and 0.5-mg. potency tablets. A simple practical manual method for the extraction of reserpine from a large number of tablets is outlined. The yellow-green fluorogenic product formed by the reaction of reserpine and nitrous acid is analyzed in the automatic analyzer system. Using excitation maximum of 390 mμ the fluoresence is measured at 510 mμ. In the colorimetric determination the absorbance is measured spectrophotometrically at 390 mμ. Using the colorimetric method, the effect of temperature on the reaction of reserpine and nitrous acid, and the effect of nitrogen gas on the recordings are investigated. In the fluorometric method there is no interference by the excipients and no effect of nitrogen gas on the recordings is noted. Further, this is more sensitive than the colorimetric method.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
