Abstract
Since the introduction of SPPS by Merrifield in the 60s, peptide chemists have considered the possibility of preparing large proteins. The introduction of native chemical ligation in the 90s and then of expressed protein ligation have opened the way to the preparation of synthetic proteins without size limitations. This review focuses on semi-synthetic strategies useful to prepare proteins decorated with spectroscopic probes, like fluorescent labels and stable isotopes, and their biophysical applications. We show that expressed protein ligation, combining the advantages of organic chemistry with the easy and size limitless recombinant protein expression, is an excellent strategy for the chemical synthesis of labeled proteins, enabling a single protein to be functionalized at one or even more distinct positions with different probes.
Highlights
IntroductionProtein structure, folding dynamics, function and interactions with other macromolecules can be widely explored and characterized by biophysical techniques such as fluorescence and NMR spectroscopies
Protein structure, folding dynamics, function and interactions with other macromolecules can be widely explored and characterized by biophysical techniques such as fluorescence and NMR spectroscopies.Spectroscopic techniques rely tightly on protein labeling strategies by which the chemical structure of a protein is modified through the introduction of biophysical probes, such as fluorophores or isotopes.A wide collection of protein labeling approaches have been developed in recent years [1,2,3,4], leading to great discoveries and innovations
Methodologies for chemical synthesis of proteins marked a breakthrough in protein and peptide chemistry, with a strong impact on chemical biology and biophysical applications [5,6]
Summary
Protein structure, folding dynamics, function and interactions with other macromolecules can be widely explored and characterized by biophysical techniques such as fluorescence and NMR spectroscopies. Spectroscopic techniques rely tightly on protein labeling strategies by which the chemical structure of a protein is modified through the introduction of biophysical probes, such as fluorophores or isotopes. A wide collection of protein labeling approaches have been developed in recent years [1,2,3,4], leading to great discoveries and innovations. The introduction of native chemical ligation (NCL). Methodologies for chemical synthesis of proteins marked a breakthrough in protein and peptide chemistry, with a strong impact on chemical biology and biophysical applications [5,6]. We review the semi-synthetic strategies employed for the preparation of labeled proteins and their spectroscopic applications
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
