Semi-Permanent Use of Indocyanine Green Latex Phantom for Fluorescence Emission.

Abstract

Testing the fluorescence emission of the vascular circulation status of the indocyanine green (ICG) fluorescence contrast agent to validate the system performance is crucial. Hence, the development of an ICG phantom is imperative, and this study proposes a method for manufacturing an ICG phantom. The ICG with an initial concentration was subjected to 0.2cc sampling through syringe(x), and an ICG (0.2cc) is diluted with silicon (Si) latex lubber (10mL) during the manufactured the phantom. The brightest fluorescence expression state is 30μM, and if it exceeded 50μM, fluorescence fading occurred and changed to a dark color. The liquid (ICG) of the concentration range is 0.003mM to 0.24mM, and the maximum fluorescence expression range is 0.005 to 0.006mM when the phantom is irradiated using a 780-nm (800mW) LED. In addition, the fluorescence emission is reduced to 0.24mM, and the fluorescence expression concentration is 10μM, 30μM, and 50μM, respectively. The decreasing of the fluorescence emission is beginning to 50μM. In this study, the proposed phantom with ICG fluorescence emission using latex lubber is proposed. In this works, the proposed phantom is improved the performance for ICG fluorescence emission. In the manufactured phantom, the phantom is used for gelatin, and the advance of phantom has easy manufacturing and long-life fluorescence emission (semipermanent) due to incorrodible material (latex lubber). To experimental results of a phantom, the ICG fluorescent contrast medium (0.055mM) is same to 30μM. Then, the 0.055mM and 30μM have high resolution and fluorescence emission status. Thus, the results are in good agreement.