Abstract
Chiral high-performance liquid chromatography (HPLC) separation of lignans with semi-micro columns of 1.0–2.0 mm inner diameter (i.d.) was established for the first time. Practical sensitivity was increased 5 to 20-fold compared with that of conventional chiral HPLC using analytical columns with 4.6 mm i.d. The semi-micro chiral HPLC system can be applied to high-sensitivity enantiomeric separation of many chiral organic compounds in addition to lignans.
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