Semi-field and indoor setups to study malaria mosquito swarming behavior

Abdoulaye Niang,Olivier Roux,Charles Nignan,Simon P Sawadogo,Olivier Gnankiné,Frédéric Tripet,B Serge Poda,Abdoulaye Diabaté,K Roch Dabiré

doi:10.1186/s13071-019-3688-0

Abstract

BackgroundThe recent resurgence of interest in sterile insect techniques to control vector mosquitoes has renewed interest in novel methods for observing mating behavior. Malarial vectors of the Anopheles gambiae complex are known to mate in swarms at specific locations at dawn and dusk. Most knowledge of mosquito swarming behavior is derived from field observations and a few experimental studies designed to assess critical parameters that affect mosquito swarming. However, such studies are difficult to implement in the field because of uncontrollable environmental factors and mosquito conditions. Here, we present two experimental setups specifically designed to analyze mosquito swarming behavior and provide evidence that swarming behavior of mosquitoes can be generated and accurately assessed under both semi-field and laboratory conditions.MethodsThe Mosquito Ecology Research Facility setup is a semi-field enclosure made of 12 compartments (10.0 × 6.0 × 4.5 m L × W × H each) exposed to ambient meteorological and lighting conditions. The laboratory setup consists of a windowless room (5.1 × 4.7 × 3.0 m) in which both environmental and mosquito conditions can be controlled. In the two setups, 300 3–6-days-old An. coluzzii virgin males were released and some swarm characteristics were recorded such as the time at which the swarm started, the number of mosquitoes in the swarm and the height. Climatic conditions in the semi-field setup were also recorded.ResultsIn both setups, An. coluzzii males displayed stereotyped and consistent swarming behavior day after day; males gradually gather into a swarm over a ground marker at sunset, flying in loops in relation to specific visual features on the ground. Although semi-field climatic conditions were slightly different from outdoors conditions, they did not impede swarming behavior and swarm characteristics were similar to those observed in the field.ConclusionsSwarm characteristics and their consistency across days provide evidences that these facilities can be used confidently to study swarming behavior. These facilities come to complement existing semi-field setups and pave the way for new experimental studies which will enhance our understanding of mating behavior but also mosquito ecology and evolution, a prerequisite for application of genetic approaches to malaria control.

Highlights

The recent resurgence of interest in sterile insect techniques to control vector mosquitoes has renewed interest in novel methods for observing mating behavior
Mating behavior is responsible for reproductive isolation and species diversification, which have important implications for vector control strategies targeting the Anopheles gambiae complex [3,4,5]
Hetero-specific swarms are rare [10]. This suggests that species-specific cues are used by males to form swarms. This mating behavior is consistent across malaria vector species prevalent in open-field habitats (An. gambiae (s.s.), An. coluzzii, An. arabiensis, An. melas, An. funestus) and across geographic regions in Africa, including Burkina Faso, Mali, Benin, Sudan, Cameroon, Gambia, Sao Tome et Principe, Mozambique, Tanzania [4, 11,12,13,14,15,16,17,18]

Summary

Introduction

The recent resurgence of interest in sterile insect techniques to control vector mosquitoes has renewed interest in novel methods for observing mating behavior. Most studies carried out during the last decades focused on female biting behavior Other behaviors in their life-cycle, Mating behavior is responsible for reproductive isolation and species diversification, which have important implications for vector control strategies targeting the Anopheles gambiae complex [3,4,5]. This suggests that species-specific cues are used by males to form swarms This mating behavior is consistent across malaria vector species prevalent in open-field habitats (An. gambiae (s.s.), An. coluzzii, An. arabiensis, An. melas, An. funestus) and across geographic regions in Africa, including Burkina Faso, Mali, Benin, Sudan, Cameroon, Gambia, Sao Tome et Principe, Mozambique, Tanzania [4, 11,12,13,14,15,16,17,18]

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Conclusion