Abstract
Enzymatic production of an important natural flavor ester, isoamyl acetate, was studied by using immobilized Candida antarctica lipase B in [bmim]PF 6 ionic liquid. The reaction was completed by removing water produced in the reaction by zeolite adsorption, and practically 100% yield was achieved. An integrated system was developed for the separation of the isoamyl alcohol‒water‒isoamyl acetate ternary azeotropic mixture, combining pervaporation and osmotic distillation. The Sulzer “Pervap 2256” flat sheet hydrophobic pervaporation membrane was found to be 100% selective for isoamyl alcohol.
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