Semi-confined compression of microfabricated polymerized biomaterial constructs

Abstract

Mechanical forces are critical parameters in engineering functional tissue because of their established influence on cellular behaviour. However, identifying ideal combinations of mechanical, biomaterial and chemical stimuli to obtain a desired cellular response requires high-throughput screening technologies, which may be realized through microfabricated systems. This paper reports on the development and characterization of a MEMS device for semi-confined biomaterial compression. An array of these devices would enable studies involving mechanical deformation of three-dimensional biomaterials, an important parameter in creating physiologically relevant microenvironments in vitro. The described device has the ability to simultaneously apply a range of compressive mechanical stimuli to multiple polymerized hydrogel microconstructs. Local micromechanical strains generated within the semi-confined hydrogel cylinders are characterized and compared with those produced in current micro- and macroscale technologies. In contrast to previous work generating unconfined compression in microfabricated devices, the semi-confined compression model used in this work generates uniform regions of strain within the central portion of each hydrogel, demonstrated here to range from 20% to 45% across the array. The uniform strains achieved simplify experimental analysis and improve the utility of the compression platform. Furthermore, the system is compatible with a wide variety of polymerizable biomaterials, enhancing device versatility and usability in tissue engineering and fundamental cell biology studies.