Semi – Automation Design Using Flow Injection Analysis System with Smart Phone for the Determination of Total Phenols in Wastewater

Abstract

Smart phone used as supporting hardware in different applications in chemical analysis are becoming increasingly important in everyday life. Rapid, easy, and straightforward analytical system flow injection analysis system for the determination of the total phenols was conducted using 4-aminoantipyrine reagent. The detection method was based on the reaction total phenols with reagent in the basic media and subsequent formation of a yellow color product. The samples or standard solutions were injection into a carrier stream to react with 4-aminoantipyrine reagent and ammonium chloride with ammonium hydroxide to give yellow color product, which was detected by spectrophotometer at 510 nm. The experimental condition such as flow rate of reagent and carrier, reagent volume, length of reaction coil and concentration of reagent were optimized. A good linear calibration curve in the range of 250-2000 mg L-1 was obtained with regression equation (y=0.0108 x + 0.3453), (R= 0.9989). The limit of detection was in the amount of 0.0112 mg L-1. The method was successfully applied for the determination of the total phenols in wastewater.