Semi-automated competitive protein binding analysis of serum thyroxine on reusable sephadex columns and its advantages over radioimmunoassay

Abstract

Competitive protein-binding analysis of serum thyroxine on small, reusable. Sephadex columns has been further studied and improved. The improved, semi-automated procedure results in reduced working time and costs. It has also been established that triiodothyronine crossreacts only 1/6 to 1/9 as well as thyroxine, and can be ignored because it represents only about. 1/80 of the total serum iodothyronine content. The economic and methodological advantages of the improved method over radioammunoassay and other displacement assays are discussed.