Abstract

Semen Evaluation of Arab Stallions during Steps of the Freezing–Thawing Protocol

Highlights

  • The development of artificial insemination with frozen – thawed semen in horses requires efforts to improve the quality of frozen sperm and minimize the inter-individual variability among stallions during the freezing process (Loomis and Graham, 2008)

  • Semen Evaluation Semen evaluation was performed following each step of the freezing and thawing protocol

  • The %MS (Figure 1) and %TAS (Figure 2) were higher in steps 1, 2, 3 and 4 as compared to those of step 5 of the freezing-thawing semen process (For %MS: respectively 63.5, 60, 63.5 and 55% vs 15.5%, p

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Summary

Introduction

The development of artificial insemination with frozen – thawed semen in horses requires efforts to improve the quality of frozen sperm and minimize the inter-individual variability among stallions during the freezing process (Loomis and Graham, 2008). During the stages of the semen freezing –thawing spermatozoa endure cellular damages, especially for plasma membrane that causes physiological and functional modifications (Pena et al, 2003). Watson (2000) reported that oxidative reaction and premature ageing occur during the cooling stage of the freezing process and contribute to sperm death. June 2016 | Volume 4 | Issue 6 | Page 279 their semen cannot support the stages of the freezing and thawing process. This study was designed to determine the semen quality of Tunisian Arab stallions during the steps of the freezing – thawing protocol

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