Abstract
Abstract Low temperature preservation of canid semen has been a subject of increasing interest among dog breeders, fur breeders and scientists. Research has focused on the use of different buffers, cryoprotectants, egg yolk additions, equilibration and cooling rates, semen packaging techniques, and freezing-thawing protocol. Some laboratories have seen a beneficial effect of post-thaw dilution, especially with vaginal semen deposition. Few reports have given fertility results on a larger scale as a basis for recommended protocol. However, it is established that artificially inseminated frozen semen can yield good fertility results (75–80% whelping rate) in dogs and in blue fox-silver fox crossbreeding, whereas pure breeding with frozen blue fox semen yields fertility results far below those from natural service. More basic research on membrane function during exposure to freezing regimes and media is called for in this species.
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