Abstract
Aim:The objective of this research is to report parameters for breeding soundness evaluation, semen extension, and cryopreservation in Rusa timorensis.Materials and Methods:Seven healthy stags were chosen for semen collection using an electroejaculator. The collections were performed twice in a breeding season between February and June 2016. Samples were collected between 2 and 3 weeks interval, collected twice for each animal. Semen was evaluated, extended, and cryopreserved using four different extenders; Andromed®, BioXcell®, Triladyl®, and a modified Tris-egg yolk combined with Eurycoma longifolia Jack.Results:R. timorensis semen characteristics according to volume (ml), color, sperm concentration (106/ml), general motility (%), progressive motility (%), and % morphology of normal spermatozoa are 0.86±0.18 ml, thin milky to milky, 1194.2±346.1 106/ml, 82.9±2.8%, 76.1±4.8%, and 83.9±4.8%, respectively.Conclusion:Semen characteristics of R. timorensis collected by electroejaculation is good allowing for cryopreservation and future artificial insemination work. The most suitable extender for Rusa deer semen is Andromed®.
Highlights
Spermatozoa had long been the center of study to humankind to bring forth the gift of life in the form of semen
Semen characteristics of R. timorensis collected by electroejaculation is good allowing for cryopreservation and future artificial insemination work
The results for Rusa deer semen characteristics according to volume, color, sperm concentration (106/ml), general motility (%), progressive motility (%), and % morphology of normal spermatozoa are 0.86±0.18, thin milky to milky, 1194.2±346.1, 82.9±2.8, 76.1±4.8, and 83.9±4.8, respectively
Summary
Spermatozoa had long been the center of study to humankind to bring forth the gift of life in the form of semen. Semen characteristics are usually used as indicators of reproductive soundness of study [1]. These parameters are not well established in Rusa deer (Rusa timorensis). A widely accepted method for safe semen collection in untrained animal is using an electroejaculator under general anesthesia [2]. This method yields acceptable semen quality in most cases. Study in semen has led to early pioneering study of artificial insemination (AI) in the veterinary field [3]. Other study in domesticated wildlife species has been shown possible with
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