Self-healing of macroscopic cracks in concrete by cellulose fiber carried microbes

Abstract

This research introduces a new approach to healing millimeter scale cracks in concrete using Lysinibaccilus Sphaericus Bacteria (LSB) encapsulated in cellulose fibers. Cracking in concrete, particularly macroscopic cracking, can cause premature structural failure and reduce its lifespan, which is a critical industry challenge. While bacteria encapsulated in cellulose fibers have been used to heal cement mortar, the studies are limited to heal much narrower cracks. In this study, we integrate LSB, known for its strong biocalcification abilities, with the protective environment of cellulose fibers, which are renewable and sustainable, for healing millimeter scale cracks in ordinary cement concrete. To understand the healing process, we firstly used a 3D-printed polymeric scaffold for preliminary observations of calcite precipitation, demonstrating the potential of bacteria-induced calcification in a controlled environment before applying these insights to concrete. We then studied the self-healing of concrete. Through mechanical testing, we identified the optimal concentration of cellulose fiber as 0.45 % by volume of mortar. Approximately 2.38 × 108 bacteria were immobilized in each gram of cellulose fibers. With cellulose fiber encapsulated LSB, the test results show up to 25 % increase in compressive strength and split tensile strength. After crack healing, the self-healing concrete still has higher mechanical strength than the undamaged control concrete. Particularly, the self-healing concrete was able to heal cracks up to 2.5 mm wide in fully wet environments and 1.5 mm wide in wet-dry conditions. This research also highlights the resilience of bacteria carried by cellulose fibers against harsh environmental conditions, including high temperatures at 160 °C, ensuring the durability and applicability of the proposed self-healing concrete in diverse climates. Integrating cellulose fibers encapsulated LSB into concrete represents a significant breakthrough in addressing the perennial problem of concrete cracking, offering a promising avenue for constructing durable and maintenance-free structures.