Abstract
The purpose of this study was to fabricate a composite structure consisting of icariin-loaded and chitosan/gelatin-sealed TiO2 nanotubes to control the drug release profile and improve the biocompatibility of the Ti substrates. Icariin was loaded onto TiO2 nanotubes and then sealed with chitosan/gelatin multilayer coatings. The characterization of each specimens were monitored by Field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), contact angle measurements (CA), X-ray photoelectron spectroscopy (XPS) and High-pressure liquid chromatography (HPLC), respectively. Additionally, the activities of osteoblasts, such as cytoskeletal and proliferative activities, and the cells' osteogenesis-related gene and protein expression on the substrates were investigated in detail. The XPS, AFM and CA results demonstrated that the composite structure of the icariin-loaded and multilayer film-sealed TiO2 nanotubes was successfully fabricated. The prepared specimens stimulated osteoblasts spreading. Furthermore, it exhibited excellent properties, promoting osteoblastic proliferation and up-regulating the expression of bone-related genes, such as osteopontin, type I collagen and osteoprotegerin, while down-regulating RANKL mRNA expression. An icariin-loaded and chitosan/gelatin multilayer-sealed TiO2 nanotube structure can modulate the bioactivity of primary osteoblasts, indicating a potential application on Ti implants to improve biocompatibility and osseointegration.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
