Self-assembled nanoparticles containing photosensitizer and polycationic brush for synergistic photothermal and photodynamic therapy against periodontitis

Mingming Zhang,Yue Wang,Enyu Shi,Liya Bai,Hanping Wang,Lujia Mao,Yinsong Wang,Changyi Li,Xiaoying Yang

doi:10.1186/s12951-021-01114-w

Abstract

BackgroundPeriodontitis is a chronic inflammatory disease in oral cavity owing to bacterial infection. Photothermal therapy (PTT) and photodynamic therapy (PDT) have many advantages for antibacterial treatment. As an excellent photosensitizer, indocyanine green (ICG) shows prominent photothermal and photodynamic performances. However, it is difficult to pass through the negatively charged bacterial cell membrane, thus limiting its antibacterial application for periodontitis treatment.ResultsIn this work, self-assembled nanoparticles containing ICG and polycationic brush were prepared for synergistic PTT and PDT against periodontitis. First, a star-shaped polycationic brush poly(2-(dimethylamino)ethyl methacrylate) (sPDMA) was synthesized via atom transfer radical polymerization (ATRP) of DMA monomer from bromo-substituted β-cyclodextrin initiator (CD-Br). Next, ICG was assembled with sPDMA to prepare ICG-loaded sPDMA (sPDMA@ICG) nanoparticles (NPs) and the physicochemical properties of these NPs were characterized systematically. In vitro antibacterial effects of sPDMA@ICG NPs were investigated in porphyromonas gingivalis (Pg), one of the recognized periodontitis pathogens. A ligature-induced periodontitis model was established in Sprague–Dawley rats for in vivo evaluation of anti-periodontitis effects of sPDMA@ICG NPs. Benefiting from the unique brush-shaped architecture of sPDMA polycation, sPDMA@ICG NPs significantly promoted the adsorption and penetration of ICG into the bacterial cells and showed excellent PTT and PDT performances. Both in vitro and in vivo, sPDMA@ICG NPs exerted antibacterial and anti-periodontitis actions via synergistic PTT and PDT.ConclusionsA self-assembled nanosystem containing ICG and polycationic brush has shown promising clinical application for synergistic PTT and PDT against periodontitis.Graphical

Highlights

Periodontitis is the sixth most prevalent disease in the world, with more than 743 million people worldwide at risk [1]
indocyanine green (ICG) was assembled with shaped polycationic brush poly(2-(dimethylamino)ethyl methacrylate) (sPDMA) to prepare ICG-loaded sPDMA nanoparticles (NPs) and the physicochemical properties of these NPs were characterized systematically
In vitro antibacterial effects of sPDMA@ICG NPs were investigated in porphyromonas gingivalis (Pg) [31, 32], one of the recognized periodontitis pathogens

Summary

Introduction

Periodontitis is the sixth most prevalent disease in the world, with more than 743 million people worldwide at risk [1]. It is a chronic inflammatory disease caused by bacteria, which eventually leads to the formation of periodontal pockets, the loss of alveolar bone and the loosening of teeth [2, 3]. Periodontitis is a chronic inflammatory disease in oral cavity owing to bacterial infection. It is difficult to pass through the negatively charged bacterial cell membrane, limiting its antibacterial application for periodontitis treatment

Methods

Results

Conclusion