Abstract
Thefeasibility of using nanomotors as active probes for lateral flow immunoassay (LFIA)is demonstrated. We synthesized Au@mSiO2@Pt Janus nanomotor, where nanolayer of Pt was deposited on the half side surface of the Au@mSiO2 nanoparticles, which can catalyze the decomposition of H2O2 to produce driving force for the nanomotor. Subsequently, the motion characteristics of the Au@mSiO2@Pt nanomotor in static fluidic environment and dynamic flow field was studied to pave the way for its practical application in lateral flow immunoassay (LFIA). At last, the Au@mSiO2@Pt nanomotor was modified with antibody and thenused as active immunoassay probe in LFIA. We chose gastric function index, pepsinogen II (PG II) and pepsinogen II (PG II), as the target analytes. The results indicated that, compared with traditional Au nanoprobe, the nanomotor-based probe can significantly improve the sensitivity by increasing the probability and efficiency of antigen and antibody binding. Alimit of detection (LOD) of 2.2ng/mL for PGI, and 2.1ng/mL for PG II was achieved. This work provides a new solution for enhancing the capability of immune detection, and we believe the nanomotor-based LFIA will have great potential in high-sensitivity point-of-care-testing in the future.
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