Abstract
A simple and cheap method to fabricate a nanocrystal ring pattern was developed by utilization of a microemulsion in this study. The mixture of polystyrene and stabilizer dichloromethane solution that contained nanocrystal aqueous solution, prepared through shaking, was applied to fabricate a reverse microemulsion. After spreading and evaporating the solvent of microemulsion on a glass slide, an ordered honeycomb film was produced, accompanied by the formation of a nanocrystal ring pattern. The nanocrystal pattern could be readily applied for immunoassays and recognition of proteins. The pattern with antibody marked by a green colored nanocrystal specifically bound with antigen labeled by a red colored nanocrystal, leading to the enhancement in red fluorescent ring pattern and decrease in green fluorescent pattern. When the unlabeled antigen was added, the green fluorescent pattern was recovered. In addition, the ring pattern with immunocomplex could selectively recognize antigen and transferrin proteins. This strategy reveals that these patterns have potential applications in biochips, biosensors, imaging analysis and so forth.
Highlights
Nanocrystals (Quantum Dots, QDs) show unique electronic and optical properties and good biocompatibility, which make these materials attractive for many potential applications including energy storage, catalysis and biosensors.[1,2,3,4] Patterning of QDs into well-de ned micro/nanoscale architecture displays potentials in multiple color light-emitting diodes, color pixels for eld-emission displays, multichannel chemical sensors, optical waveguides, and so on.[5]
The pattern with antibody marked by a green colored nanocrystal bound with antigen labeled by a red colored nanocrystal, leading to the enhancement in red fluorescent ring pattern and decrease in green fluorescent pattern
Transferrin, which does not bind to antibody IgG-QDs, reveals that there is no in uence on the Forster resonance energy transfer (FRET) process between bovine serum albumin (BSA)-QDs and IgG-QDs
Summary
Nanocrystals (Quantum Dots, QDs) show unique electronic and optical properties and good biocompatibility, which make these materials attractive for many potential applications including energy storage, catalysis and biosensors.[1,2,3,4] Patterning of QDs into well-de ned micro/nanoscale architecture displays potentials in multiple color light-emitting diodes, color pixels for eld-emission displays, multichannel chemical sensors, optical waveguides, and so on.[5]. The QDs ring pattern has been prepared by a microemulsion method through the incorporation of QDs into the cavities on the polymer lm.
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