Abstract
DcMYB1 is a member of the R2R3-type MYB protein family in carrot and can activate expression of the DcPAL1 gene induced by stresses such as the dilution effect, UV-B irradiation and elicitor treatment. To understand the regulatory mechanism of DcMYB1 we screened cDNA for proteins interacting with DcMYB1 as bait in a yeast two-hybrid system. Several positive cDNA, which were isolated as prey, encoded DcMYB1, indicating that DcMYB1 could interact with itself. The interaction of full-length DcMYB1 protein with partial DcMYB1 amino acid sequences derived from DcMYB1 cDNA deletion mutants was observed in yeast and carrot protoplasts, suggesting the self-interaction of R2R3-type MYB protein of DcMYB1 in vivo.
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