Abstract
Abstract The objective of this study was to characterize the parents and respective populations of apple trees regarding S-alleles to confirm their genealogy and to evaluate the efficiency of the molecular markers used. Sixteen specific sets of primers were used for identification of apple S-alleles by PCR. Two segregating populations of the Epagri Apple Breeding Program resulting from crosses between ‘Fred Hough’ × ‘Monalisa’ and ‘M-11/00’ × ‘M-13/91’ were evaluated. The expected segregations are 1:1:1:1 for full compatibility and 1:1 for semi-compatibility, which can be confirmed by the X2 test. The ‘Fred Hough’ (S5S19) × ‘Monalisa’ (S2S10) cross proved to be fully compatible; and two triploids were identified among the hybrids as well. The ‘M-11/00’ (S3S19) × ‘M-13/91’ (S3S5) cross was characterized as semi-compatible based on DNA markers, and the segregation of the S-alleles in the hybrids was 1:1, as expected. The segregation of the DNA markers occurred together with their respective S-alleles: S2, S3, S5, S10, and S19. Thus, characterization of the S-alleles not only allowed identification of compatibility between parents but also identified contaminations in segregating populations.
Highlights
Materials and methodsGametophytic self-incompatibility is present in the reproductive process of several Malus species, including Malus × domestica Borkh. (BROOTHAERTS, 2003)
When the primer is adapted to a region distant from the gene of interest, distortions may occur in the expected frequency of alleles in the segregating populations, resulting in false negative or positive results
‘Fred Hough’ (S5S19) × ‘Monalisa’ (S2S10), 49 apple trees exhibited one of the genotypes expected from this fullycompatible cross (S2S5, S5S10, S2S19, and S10S19)
Summary
Materials and methodsGametophytic self-incompatibility is present in the reproductive process of several Malus species, including Malus × domestica Borkh. (BROOTHAERTS, 2003). E-mail: thyanalays@hotmail.com(ORCID 0000-0003-3337-6314) 2Agronomist, D.Sc. Researcher, Epagri – Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina, Estação Experimental de Caçador, Caçador-SC, Brazil. E-mail: fernando.hawerroth@embrapa.br(ORCID 0000-0003-1138-2457) 4Agronomist, D.Sc. Researcher, Epagri - Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina, Estação Experimental de Caçador, Caçador-SC, Brazil.
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