Abstract
We used a baculovirus expression system to express fusion proteins of HCV core, RGD (Arg-Gly-Asp) peptide, and IFN-α2a fragments in Sf9 cells. Western blotting and electron microscopy demonstrate that HCV core, peptides RGD, and IFN-α2a fusion proteins assemble into 30 to 40 nm nano-particles (virus-like particles, VLPs). Xenograft assays show that VLPs greatly reduced tumor volume and weight with regard to a nontreated xenograft. Migration and invasion results show that VLPs can inhibit the migration and invasion of the breast cancer cells MDA-MB231. This study will provide theoretical and experimental basis for the establishment of safe and effective tumor-targeted drug delivery systems and clinical application of VLPs carrying cell interacting cargo.
Highlights
According to the World Health Organization (WHO), cancer is one of the leading causes of death worldwide
This study provides theoretical and experimental basis for the establishment of safe and effective tumor-targeted drug delivery systems and clinical application of Virus-like particles (VLPs)
MDA-MB231 human breast cancer cells, HCT116 human colon cancer cells, and 293 T human embryonic kidney cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum, 100 U/ml penicillin G, and 100 μg/ml streptomycin, at 37°C under 5% CO2, provided by Wuhan Institute of Virology, Chinese Academy of Sciences, China Center for Type Culture Collection (CCTCC, Wuhan, China)
Summary
According to the World Health Organization (WHO), cancer is one of the leading causes of death worldwide (http:// www.who.int/mediacentre/factsheets/fs297/en/index.html). Cancer control has become a global health strategic focus. Treatment of malignant tumors traditionally involves a combination of surgery, radiation therapy, and chemotherapy. Surgery and radiation therapy are effective in addressing the local tumor; chemotherapy, carries severe toxicity due to lipid solubility and high therapeutic doses required for most cancers (>70%) [1]. With these therapeutic limitations, combination therapy has received close attention in the recent years. The addition of interferon (IFN) has become one of the most common additions to combination therapies
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