Abstract
The Atractylodes macrocephala polysaccharide (AMP) was extracted by water extracting-alcohol precipitating method and further purified by DEAE column. After that, the polysaccharides were modified by nitric acid-sodium selenite method, and nine kinds of selenizing AMPs (sAMPs) were obtained, namely, from sAMP1 to sAMP9. AMP and sAMP were characterized using FTIR spectrometry. Then their antioxidant activities in vitro were measured by free radical-scavenging test. Among these, sAMP6 presented the strongest antioxidant effect. For the test in vivo, the chickens at day 14 vaccinated with ND vaccine were repeatedly vaccinated at day 28. The chickens in sAMP and AMP were injected respectively with 1 mg of sAMP6 and AMP and, in vaccination control (VC) and BC groups, injected with equal volume of normal saline. Respectively, after the first vaccine, on days 7, 14, 21, and 28, the serum GSH-Px and SOD activities and MDA content were determined. The results suggested that sAMP6 could significantly promote GSH-Px and SOD activities and decrease MDA content. All these results indicated that selenylation modification could significantly enhance the antioxidant activity of AMP.
Highlights
Selenium is a trace element necessary for life [1] and an important food source of antioxidants
The aim of this study was to observe whether selenylation modification could enhance the antioxidation activity of Atractylodes macrocephala polysaccharide, so as to select the selenizing polysaccharide with strongest activity and provide the theoretical basis for the development of novel antioxidants
In the selenylation modification of polysaccharide, the nitric acid-sodium selenite method is one with most reports. It possesses a series of advantages such as simple process, less pollution and strong feasibility [20]. This method was chosen as the selenylation modification of Atractylodes macrocephala polysaccharide (AMP) and the immuneenhancing activities of selenizing AMPs (sAMPs) were compared in the published research in our previous study [12]
Summary
Selenium is a trace element necessary for life [1] and an important food source of antioxidants. Chinese medicine polysaccharide has antioxidant, immune enhancement, antitumor, antiradiation effect and so on. Selenium-polysaccharide is organic selenium, as a compound of selenium combined with polysaccharides; it can play a dual function of selenium and polysaccharides [6] and effectively improve the bioavailability of selenium, significantly reducing toxicity and side effects more than inorganic selenium. It can be used as a safe, effective, healthy nutrient of selenium [7]. Researchers have been exploring the chemical modification method in order to obtain more seleniumpolysaccharide or selenizing polysaccharide [8]
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