Abstract
Aim: To evaluate the effect of Selenium-enriched Lactobacillus acidophilus (Se-enriched L. acidophilus) on dextran sulfate sodium (DSS)-induced colitis in mice.Methods: Mice were randomly divided into four groups: a control group, a control + Se-enriched L. acidophilus group, a chronic colitis group, and a chronic colitis + Se-enriched L. acidophilus group (n = 10 each group). The mice were sacrificed on the 26th day. The disease activity index, survival rates, and histological injury score were determined. Cytokines produced by lamina propria lymphocytes (LPLs), the selenium (Se) concentrations in serum and colon tissue and the mouse intestinal microbiota were evaluated.Results: Se-enriched L. acidophilus can improve histological injury and the disease activity index in mice with chronic colitis and reduce IL-1β, IL-6, IL-12p70, TNF-α, IL-23, IFN-γ, IL-17A, and IL-21 (P < 0.05) and increase IL-10 (P < 0.05) expression levels. Moreover, Se-enriched L. acidophilus can increase the β diversity of intestinal microbiota in mice with chronic colitis, significantly reduce the relative abundance of Lactobacillus and Romboutsia (P < 0.05), and significantly increase the relative abundance of Parasutterella (P < 0.05).Conclusions: Se-enriched L. acidophilus can improve DSS-induced chronic colitis by regulating inflammatory cytokines and intestinal microbiota.
Highlights
Inflammatory bowel disease (IBD) is a chronic recurrent inflammatory disease of the intestine that mainly includes two forms, ulcerative colitis (UC) and Crohn’s disease (CD) [1], and its prevalence is increasing annually [2]
The effect of Se-enriched L. acidophilus on dextran sulfate sodium (DSS) colitis was compared by the differences in survival rate, DAI score and colon histology between the two groups
Detection of the cytokine concentrations in the supernatant revealed that IL-1β, IL-6, IL-12p70, TNFα, and IL-23 were significantly decreased in the chronic colitis + Se-enriched L. acidophilus group compared with the chronic colitis group (P < 0.05)
Summary
Inflammatory bowel disease (IBD) is a chronic recurrent inflammatory disease of the intestine that mainly includes two forms, ulcerative colitis (UC) and Crohn’s disease (CD) [1], and its prevalence is increasing annually [2]. There are a Colitis and Selenium-Enriched Lactobacillus acidophilus large number of microbiota in the human intestine, which has an important impact on the human body, and disorders of the intestinal flora are considered to be closely related to the occurrence and development of IBD [4]. L. acidophilus can alleviate the pain caused by osteoarthritis and delay the progression of osteoarthritis by reducing the destruction of cartilage and inhibiting the production of proinflammatory cytokines [11]. It has a certain relieving effect on type 2 diabetes [12]. Evodiamine can relieve dextran sulfate sodium (DSS)-induced colitis by increasing L. acidophilus in the intestine [15]
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