Selenium-Containing Nanobiocomposites of Fungal Origin Reduce the Viability and Biofilm Formation of the Bacterial Phytopathogen Clavibacter michiganensis subsp. sepedonicus

Abstract

The effect of selenium biocomposites obtained from medical macrobasidiomycetes Ganoderma lucidum, Grifola umbellata, Laetiporus sulphureus, Lentinula edodes, and Pleurotus ostreatus on the viability of the phytopathogenic gram-positive bacteria Clavibacter michiganensis subsp. sepedonicus (Cms) and its ability to form biofilms has been studied. A decrease in the viability of the bacterial cells as a result of incubation with biocomposites is shown. The determining effect of the selenium component of the composites on the studied biological activity is investigated. The dependence of the antimicrobial action of selenium-containing experimental samples on the biological species of the fungus is revealed. Biocomposites based on extracellular metabolites of Lentinula edodes and Ganoderma lucidum possess maximal activity. When biopolymer samples of fungal origin are added to the bacterial suspension, the ability of Cms to form biofilms differs depending on the type of biocomposite; it decreases significantly in some cases.