Abstract
Selenium is a well-known essential element with important roles in human reproductive health mainly due to its antioxidant character. This study aimed to investigate the potential role of selenoproteins on gut microbiota and male reproductive health. A new assay for the absolute quantification of selenoproteins in testicular tissue based on two dimensional chromatography with inductively coupled plasma mass spectrometry was performed for the first time. The gut microbiota profile was obtained by 16S rRNA gene sequencing. Numerous associations were found between testicular selenoproteins and gut microbiota (e.g. Mucispirillum, related with sperm activity and testosterone, was associated with glutathione peroxidase (GPx) and selenoalbumin (SeAlb), while Escherichia/Shigella, related to sex hormones, correlated with GPx, selenoprotein P (SelP) and SeAlb). The effects of Se-supplementation on testicular selenoproteins only occur in conventional mice, suggesting a potential selenoproteins-microbiota interplay that underlies testicular function. The selenoproteins GPx and SelP have been quantified for the first time in the testicles, and the novel identification of SeAlb, a protein with nonspecifically incorporated Se, is also reported. These findings demonstrate the significant impact of Se-supplementation on gut microbiota and male reproductive health. In addition, the analytical methodology applied here in selenoprotein quantification in testicular tissue opens new possibilities to evaluate their role in gut microbiota and reproductive health axis.
Highlights
Spermatogenesis is mainly mediated by two selenoproteins, namely phospholipid hydroperoxide glutathione peroxidase (PHGPx/GPx4) related to sperm quality and male fertility and SELENOP, a plasma protein required for Se supply to the gonads where it is used as a reservoir of Se9
The aim of this work is to investigate the potential role of selenoproteins in the gut microbiota-reproductive health axis
The absolute quantification of selenoproteins by intact protein analysis will be performed using a metallomic approach based on inductively coupled plasma mass spectrometry (ICP-MS), a methodology used for analyses of serum, p lasma[24] and the liver[25]
Summary
Spermatogenesis is mainly mediated by two selenoproteins, namely phospholipid hydroperoxide glutathione peroxidase (PHGPx/GPx4) related to sperm quality and male fertility and SELENOP, a plasma protein required for Se supply to the gonads where it is used as a reservoir of Se9. The aim of this work is to investigate the potential role of selenoproteins in the gut microbiota-reproductive health axis. For this purpose, the absolute quantification of selenoproteins by intact protein analysis will be performed using a metallomic approach based on inductively coupled plasma mass spectrometry (ICP-MS), a methodology used for analyses of serum, p lasma[24] and the liver[25]. The absolute quantification of selenoproteins by intact protein analysis will be performed using a metallomic approach based on inductively coupled plasma mass spectrometry (ICP-MS), a methodology used for analyses of serum, p lasma[24] and the liver[25] To this end, mice testicular selenoproteome has been determined after Se-supplementation of conventional mice and mice with microbiota depleted by antibiotics. The total metal content in testicles has been measured to evaluate the possible impact of Sesupplementation and microbiota on the homeostasis of elements in testicles, as well as their traffic
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
