Selenium nanoparticles with photocatalytic properties synthesized by residual activated sludge

Abstract

The treatment and disposal of residual activated sludge is a worldwide problem and the research on its reuse is still only in the earliest stages. Selenite is a toxic pollutant, while selenium nanoparticles (SeNPs) are environmentally friendly and have promising application prospects. At present, the reduction mechanism of selenite under the complex system is still poorly understood. In order to explore the mechanism of SeNPs synthesis by activated sludge resource utilization, SeNPs were synthesized by activated sludge extracts of domestic sewage (DSeNPs) and coking sewage (CSeNPs), respectively. The synthesis process, zeta potential and morphology size of SeNPs were changed by pH value, extract concentration and extract composition. Under the same synthesis conditions, the morphologies of DSeNPs and CSeNPs were mainly spherical and pseudo–spherical, while CSeNPs also contained pseudo-rod shape particles. The sizes and crystal grains of CSeNPs were smaller than those of DSeNPs. Compared with DSeNPs, a specific protein (~35 kDa) was found on the surface of CSeNPs using SDS-PAGE. By analyzing the fluorescence images of the two SeNPs, it was found that the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces were obviously different (P < 0.05). The present study demonstrated that proteins, polysaccharides, humic-like and fulvic acid-like substances cooperated in the formation and stabilization of SeNPs. Furthermore, CSeNPs (bandgap: 1.68 eV) had more desirable photocatalytic performance than DSeNPs (bandgap: 1.84 eV). Under the light condition, CSeNPs could degrade Rhodamine B faster without adding hydrogen peroxide. This experiment provided a new insight into the resource utilization of activated sludge and a reference for the synthesis of nanometer selenium with excellent performance.