Selenium enriched yeast and Gum Arabic combination attenuate oxidative liver damage via suppression of oxidative stress, inhibition of caspase-3 and pro-inflammatory genes expression in carbon tetrachloride-intoxicated rats

Abstract

In this study we investigated the potential synergistic effects of Gum Arabic (GA) and Selenium enriched yeast (SY) combination on the carbon tetrachloride (CCl4) -caused oxidative damage in the liver of rats. The rats were separated into 5 groups (8 animals per group) and subjected to GA, SY and GA + SY diets following CCl4 injection. The results showed that GA + SY supplementation demonstrated higher protective antioxidative properties than the supplementation of either GA or SY alone, as manifested by lesser activities of serum alkaline phosphatase (ALP), serum transaminase, hepatic malondialdehyde (MDA), and augmented the hepatic activities of superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GSH-Px) consistent with up-regulation of antioxidant-related genes including glutathione peroxidase 1 (GPX1), superoxide dismutase (SOD1), and heat shock protein 70 (HSP70) in CCl4-intoxicated rats. Furthermore, GA + SY supplementation revealed mild pathological damage and effectively attenuated expression levels of the proinflammatory genes including interleukin-6 (IL-6), interleukin-1β (IL-1β), and tumor necrosis factor- α (TNF-α). Moreover, GA + SY supplementation alleviated CCl4-induced hepatocytes apoptosis via suppression of caspase-3 expression. We concluded that GA + SY combination displayed strong protective and antioxidative effects against hepatotoxic injury caused by CCl4 in rats via its ability to suppress the oxidative stress, inhibit the caspase-3 and pro-inflammatory genes expression.