Abstract
Dietary intake of the essential trace element selenium (Se) regulates expression of genes for selenoproteins, including the gene for type I iodothyronine 5′-deiodinase. However, regulation of these genes does not account for all of Se's biological effects. To define additional mechanisms for Se's effects, these experiments aimed to identify other genes also regulated by Se intake in rats. Weanling male Sprague-Dawley rats were fed a Torula yeast-based Se-deficient diet, or the same diet supplemented with 0.5 mg Se/kg diet for 13 weeks. Differential display of mRNA was used to identify cDNAs reverse transcribed from mRNAs present at different levels in the livers of the two groups of rats. Northern blots, probed with these cDNAs, were used to quantitate differences in mRNA levels between the two dietary groups. Those cDNA probes detecting differential expression in Northern blots were sequenced. Homology searches in DNA sequence data bases were conducted to identify the differentially expressed genes. This analysis revealed that mRNA levels for transthyretin and citrate transport protein are both reduced in the livers of Se-deficient rats. Reductions averaged 44.7% (p=0.0009) for transthyretin and 42.8% (p=0.0032) for citrate transport protein. These results suggest a coordinated regulation of thyroid hormone metabolism in rats by dietary Se intake.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
