Abstract

Environmental bacterial isolates play a very important role in bioremediation of metals and toxic metalloids. A bacterial strain with high selenite (SeO32-) tolerance and reducing capability was isolated from electronic waste dump site in Banaras Hindu University, Varanasi, India. Based on 16S rRNA sequencing and BLAST search, this bacterial isolate was identified as Bacillus paramycoides and designated as strain MF-14. It tolerated Sodium selenite up to 110 mM when grown aerobically in LB broth and reduced selenite into elemental selenium (Se0) significantly within 24h with concomitant biosynthesis of selenium nanoparticles as clearly revealed by brick red precipitate and specific surface plasmon resonance peak at 210nm using UV-Visible spectrophotometer. Scanning electron microscopy (SEM) analysis of this bacterial strain exposed to 1mM and 5 mM selenite also demonstrated morphological alterations as cell enlargement due to accumulation and bioprecipitation of elemental selenium (Se0). The FTIR analysis clearly demonstrated that functional groups present on the surface of biogenic selenium nanoparticles (SeNPs) play a significant role in the stabilization and capping of SeNPs. Furthermore, these SeNPs were characterized using spectroscopic analysis involving Dynamic light scattering, zeta potential, XPS, FTIR, XRD and Raman spectroscopy which clearly revealed particle size 10-700nm, amorphous nature, stability as well as it's oxidation state. The biochemical studies have demonstrated that membrane bound reductase enzyme may be responsible for significant reduction of selenite into elemental selenium. Therefore, we may employ Bacillus paramycoides strain MF-14 successfully for bioremediation of selenite contaminated environmental sites with concomitant green synthesis of SeNPs.

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