Abstract
Fatty acid incorporation into discrete acylglycerol (AG) pools during esterification reactions with glycerol was analyzed for processes mediated by Rhizomucor miehei and Burkholderia cepacia lipases. Multicompetitive mixtures of saturated FA (C4–C18 even chain) and oleic acid (C18:1) were reacted with glycerol and the temporal incorporation of FA into mono-, di- and triacylglycerol pools (MAG, DAG, TAG) was quantified by relative selectivity constants and dimensionless fatty acid partitioning coefficients. Both analyses revealed a general fidelity of FA selectivity during FA incorporation into each AG pool for each lipase, and little evidence of preferential incorporation of C8–C18 FA into an individual AG pool was obtained. Exceptions were noted for C4–6 FA and C18:1 FA which became selectively diminished and enriched, respectively, in the DAG and TAG pools. The observation that FA selectivity may be generally conserved throughout the sequential steps of TAG assembly may simplify the design of processes designed to yield specifically structured TAG using these lipases.
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