Selectively probing ferric ions in aqueous environments using protonated and neutral forms of 7-azaindole as a multiparametric chemosensor.

Abstract

7-azaindole (7AI) dimer is a model molecule for DNA study and understanding the mutagenic behavior based on the excited-state proton transfer process in hydrogen-bonded networks. The neutral and protonated forms of 7AI monomer with significant fluorescence (FL) intensity fit the fluorescent sensor strategy to recognize selective metal ions. Out of several metal ions (Fe3+, Al3+, Fe2+, Pb2+, Ba2+, Ni2+, Zn2+, Mg2+, Ca2+, Cu2+, Hg2+ and Cd2+), the absorption, fluorescence and fluorescence lifetime of 7AI in the aqueous medium are selectively sensitive to the ferric (Fe3+) ions. The absolute value of absorption intensity increases linearly with concentration of a particular metal ions. FL intensity of both the forms of 7AI decreases gradually with Fe3+ ions and trails the linear Stern-Volmer relation. The formation of non-fluorescent complexes was confirmed with Benesi-Hildebrand and Job plots, along with FL and FL decays. The FL lifetime of the protonated form of 7AI, which is 0.83 ± 0.01ns, is nearly constant with Fe3+ ions concentrations, confirming the static quenching mechanism. The limit of detection (LoD) of Fe3+ ions over the long range of 16-363µM for the neutral and protonated forms of 7AI is 0.46 ± 0.02 and 0.49 ± 0.02µM, respectively, estimated using FL spectra. Additionally, the linear plot of absorbance with Fe3+ ions of both the forms of 7AI can also act as a calibration curve with very close LoDs, as obtained by FL spectra. Thus, the multi-parameters-based probe for detecting the Fe3+ ions over long-range in real aqueous environments with operational, high sensitivity, fast response (< 5s), and good selectivity (over 12 metal ions) is undoubtedly a superior approach over other methods.