Abstract
We have previously shown that freshly collected guinea-pig alveolar macrophages (AM) showed low levels of sPLA2 activity. This activity increased progressively in cultured AM, to reach levels 40–50-fold above basal values within 20 h of adhesion. This increase was abrogated in the presence of actinomycin D or cycloheximide and in experimental conditions that prevent the adherence of AM, suggesting that AM produce an sPLA2 through RNA and protein synthesis-dependent processes induced by their adherence. Adherence of AM induced selectively the synthesis of sPLA2 since the level of the cPLA2 activity did not change significantly with the duration of adhesion. In contrast to the cPLA2 activity, which was mainly recovered in the soluble fraction of AM sonicates, the sPLA2 activity was located in the membrane fractions. The increase observed in the sPLA2 activity occurred independently from the release of AA by stimulated AM, suggesting that these two processes are distinct.
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