Abstract
Three simple and selective spectrophotometric methods were developed for the quantitative determination of Fenoterol in pure forms as well as in its pharmaceutical formulation. Method [A] Involves the coupling of the drug as phenolic compound with the diazonium salts of four amines, namely, Benzocaine (BZC), sulphadiazine (SDZ), sulphacetamide (SCM) and sulphanilic acid (SPA) forming red azodyes absorbed at 526, 514, 512 and 513 nm, respectively. Beer's law was obeyed in the concentration ranges of 10-70, 6-42, 4-28 and 3.5-24.5 μg ml-1 for the four reagents, respectively. Method [B] is based on reaction of Fenoterol with cobalt thiocyanate, where by a sparingly soluble blue ion-pair complex is formed. This complex is extracted by toluene and spectrophotometrically measured at 619 nm. Good linearity was obtained in the range of 10-70 μg ml-1. Method [C] is based on the reduction of iron (III) by Fenoterol in acid medium and subsequent interaction of iron (II) with ferricyanide to form Prussian blue. The product exhibits absorption maximum at 730 nm. Beer's law was obeyed in the concentration range of 1.5–10.5 μg ml-1. The reaction conditions for described methods were studied and optimized. The proposed methods were applied to the determination of Fenoterol in pharmaceutical formulation and the results demonstrate that the methods are equally accurate and precise as the reported methods found from the t and F values. The reliability of the methods was established by recovery studies using standard addition technique.
Highlights
Fenoterol (Fig. 1) is a direct acting sympathomimetic agent with predominantly betaadrenergic activity and a selective action on β2 receptors
In a series of 25 ml volumetric flasks, different volumes (1-10 ml) of BZC solution (4.12 x 10-5 M), SDZ solution (2.47 x10-5M), SCM solution (1.64 x 10-5 M) and sulphanilic acid (SPA) solution (1.44 x10-5 M) were mixed with 1 ml of 0.1 N hydrochloric acid and 2 ml of sodium NaNO2 solution (1%) the mixture were left to stand for 10 minutes, 1 ml of Fenoterol solution (4.12 x 10-5 M), (2.47 x10-5M), (1.64 x 10-5 M) and (1.44 x 10-5 M), respectively were added to each flask followed by 1 ml of 0.2 N sodium hydroxide, again stay for 5 minute, complete the volume to the mark with water and measure the absorbencies at their corresponding λmax
A (Diazo-coupling technique): The utility of diazotized different amines BZC, SDZ, SCM and SPA as chromogenic reagents for the determination of the phenolic drug was investigated in the present study
Summary
Fenoterol (Fig. 1) is a direct acting sympathomimetic agent with predominantly betaadrenergic activity and a selective action on β2 receptors. It is used as bronchodilator, with its bronchodilating action being relatively more prominent than its effect on the heart. It is used in the treatment of bronchial asthma, prevention of exercise-induced bronchspasm and in the management of premature labour (Kathleen, 1999). The aim of the present study is to develop new, simple, and accurate quantitative methods for determination of fenoterol in both pure and pharmaceutical forms
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