Abstract

New selective and sensitive spectrophotometric and spectrofluorometric methods have been developed and validated for the determination of amantadine hydrochloride (AMD) in capsules and plasma. The methods were based on the condensation of AMD with 1,2-naphthoquinone-4-sulphonate (NQS) in an alkaline medium to form an orange-colored product. The spectrophotometric method involved the measurement of the colored product at 460 nm. The spectrofluorometric method involved the reduction of the product with potassium borohydride, and the subsequent measurement of the formed fluorescent reduced AMD-NQS product at 382 nm after excitation at 293 nm. The variables that affected the reaction were carefully studied and optimized. Under the optimum conditions, linear relationships with good correlation coefficients (0.9972–0.9974) and low LOD (1.39 and 0.013 μg mL−1) were obtained in the ranges of 5–80 and 0.05–10 μg mL−1 for the spectrophotometric and spectrofluorometric methods, respectively. The precisions of the methods were satisfactory; RSD ≤2.04%. Both methods were successfully applied to the determination of AMD in capsules. As its higher sensitivity, the spectrofluorometric method was applied to the determination of AMD in plasma; the recovery was 96.3–101.2 ± 0.57–4.2%. The results obtained by the proposed methods were comparable with those obtained by the official method

Highlights

  • Amantadine hydrochloride (AMD), Scheme 1, is an antiviral agent used against infection with influenza type A virus and to ameliorate symptoms when administered during the early stages of infection as well as in the management of herpes zoster [1]

  • Derivatization of AMD was attempted in the present study for the development of both spectrophotometric and spectrofluorometric methods for its determination

  • The present study described the use of NQS reagent for the development of selective, sensitive, and accurate spectrophotometric and spectrofluorometric methods for the determination of AMD in bulk, capsules, and plasma

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Summary

Introduction

Amantadine hydrochloride (AMD), Scheme 1, is an antiviral agent used against infection with influenza type A virus and to ameliorate symptoms when administered during the early stages of infection as well as in the management of herpes zoster [1]. It has mild anti-Parkinsonism activity and it has been used in the management of Parkinsonism, mainly in the early disease stage and when the symptoms are mild. The present study was devoted to explore NQS as a derivatizing reagent in the development of selective and sensitive spectrophotometric and spectrofluorometric methods for the determination of AMD in capsules and plasma

Experimental
Preparation of Standard and Sample Solutions
General Recommended Procedure
Results and Discussion
Method Development
Method Validation
Method
Conclusions
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