Abstract

Rhodamine–thiophene conjugate (L) has been synthesized and characterized by 1H NMR, FTIR and mass spectra. L shows a large enhancement in emission intensity in presence of Hg2+. Moreover, naked eye color of L becomes intense red in presence of Hg2+. The lowest detection limit for Hg2+ is 1×10−9M in HEPES buffer (0.1M in EtOH/water, 1/1, v/v, pH 7.4). Hg2+ induced chelation enhanced fluorescence (CHEF) is associated with spirolacram ring opening of the rhodamine unit. Trace level intracellular Hg2+ is visualized under fluorescence microscope.

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