Abstract

This study was undertaken to assess the effectiveness of alternative methods and durations of REM sleep deprivation with rats, and to explore the feasibility of suitable controls. A total of 24 chronically implanted rats on an 8 h schedule were monitored for 3 consecutive days to determine basal amount of REM sleep. All animals were then subjected to one of four control or experimental conditions for either 3 or 6 days, followed by 3 consecutive days of recovery sleep. Water tank deprivation, using a small pedestal, appeared the equivalent of a more traditional arousal method of depriving REM sleep. Neither control arousals during NREM sleep nor the water tank control procedure utilizing a large pedestal, produced substantial deprivation. Negligible differences were found between 3 and 6-day deprived rats in compensation for lost REM time on recovery days, suggesting a ‘plateau’ effect. Other measures suggest that ‘intensity’ changes may account for additional compensation beyond the plateau of increased REM sleep time.

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