Selective recovery of the intracellular enzyme using hydrodynamic cavitation: Scalable approach

Abstract

Hydrodynamic cavitation technology is a promising technology for the large-scale recovery of periplasmic enzymes. In the present work, we have studied a selective, scalable, and energy-saving approach for the recovery of the alcohol dehydrogenase (ADH) enzyme from the cytoplasm of yeast cells. The hybrid strategy of hypoosmotic alkaline pretreatment (0.001 M, 8 pH, 6h) followed by the enzyme recovery using hydrodynamic cavitation (6 bar, 5 passes) increases the selective release (34.81 U/mg) up to 21.59 times when it is compared with the recovery of the enzyme using hydrodynamic cavitation without pretreatment ((1.612 U/mg, 6 bar, 55 passes). The hypoosmotic alkaline pretreatment (0.001 M, 8 pH, 9h) followed by enzyme recovery using hydrodynamic cavitation (6 bar, 5 passes) increases the specific yield (energy efficiency; U/kJ) up to 58.62 times (1793.27 U/kJ, 6 bar, 5 passes) as compared to enzyme recovery using hydrodynamic cavitation without the pre-treatment (30.59 U/kJ, 6 bar, 35 passes).