Abstract
Therapeutic effects of adult stem-cell transplantations are limited by poor cell-retention in target organs, and a reduced potential for optimal cell differentiation compared to embryonic stem cells. However, contemporary studies have indicated heterogeneity within adult stem-cell pools, and a novel culturing technique may address these limitations by selecting those for cell proliferation which are highly functional. Here, we report the preservation of stemness in human adipose-derived stem cells (hASCs) by using microgravity conditions combined with microspheres in a stirred suspension. The cells were bound to microspheres (100−300 μm) and cultured using a wave-stirring shaker. One-week cultures using polystyrene and collagen microspheres increased the proportions of SSEA-3(+) hASCs 4.4- and 4.3-fold (2.7- and 2.9-fold increases in their numbers), respectively, compared to normal culture conditions. These cultured hASCs expressed higher levels of pluripotent markers (OCT4, SOX2, NANOG, MYC, and KLF), and had improved abilities for proliferation, colony formation, network formation, and multiple-mesenchymal differentiation. We believe that this novel culturing method may further enhance regenerative therapies using hASCs.
Highlights
The total number of cells, their viability, and stage-specific embryonic antigen-3 (SSEA-3)-positivity of viable cells were assessed in normal human adipose-derived stem cells (hASCs) and in five kinds of stress-treated hASCs
No significant differences were detected in the overall numbers of SSEA-3(+) cells between stress-treated hASCs and normal hASCs, with the exception of the mechanostress group, which showed a significantly lower number of SSEA-3(+) cells (p < 0.01) (Figure 1B,C)
(1.7 × 103 ) compared to the controls (1.3 × 103 ), with no statistically significant difference. These results suggest that exposure to severe stress can concentrate SSEA-3(+) hASCs through the selective depletion of SSEA-3(−) cells, but such stress can inhibit cell proliferation
Summary
Human adipose-derived stem cells (hASCs) are isolated therapeutic stem cells from adipose tissue. They have increasingly been used to ameliorate a broad range of refractory diseases, including irradiated tissue [1], ischemic wounds [2], and diabetic ulcers [3]. Their clinical efficacy has been limited because the long-term survival of transplanted hASCs is diminished by environmental stress [4,5,6]. The use of embryonic stem cells is limited by ethical issues [12]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
