Abstract
A selective medium was developed for the dilution plate isolation of Mycoleptodiscus terrestris from natural soils and sediments from aquatic environments. The ingredients per liter of the selective medium are as follows: KH(2)PO(4), 0.5 g; MgSO(4) 7H(2)O, 0.5 g; dextrose, 10.0 g; peptone, 5.0 g; chloramphenicol, 0.25 g; rose bengal, 50 mg; oxgall, 5.0 g; Terraclor (pentachloronitrobenzene, 75% active ingredients), 0.5 g; agar, 15.0 g. After autoclaving, the following ingredients were aseptically added: sorbic acid (0.7% autoclave-sterilized aqueous solution), 5.0 ml; Subdue (25.1% emulsion of metalaxyl), 0.5 ml; Truban (40.7% suspension of etridiazol), 0.05 ml. The colony-restrictive properties of this medium enabled its use in the drop plate method, originally developed for viable counts of bacteria. Alfalfa sprouts as baits were not suitable for quantitative recovery of the fungus, although 5% of alfalfa sprouts were infected with M. terrestris when incubated on soil containing 1.5 x 10 CFU/g.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
