Abstract
Selective media for ice nucleation-active (INA) Xanthomonas campestris and INA Erwinia ananas were developed for studying their behavior on tea buds in respect to frost damage. The selective medium developed for INA X. campestris abbreviated SGNC was a synthetic medium containing NH4H2PO4 1g, MgSO4⋅7H2O 0.2g, KCl 0.2g, glycogen 10g, novobiocin 100mg, cycloheximide 100mg, agar 15g, and distilled water 1, 000ml, pH6.8. The selective medium for INA E. ananas abbreviated NSVC was nutrient agar (yeast extract 5g, peptone 10g, agar 15g, distilled water 1, 000ml, pH 6.8) supplemented with NaCl 50g, vancomycin 100mg, and cycloheximide 100mg. The plating efficiencies of these selective media were almost same as those of nutrient agar medium. INA X. campestris formed separate colonies 0.5-1.0mm in diameter 3 to 4 days after inoculation, whereas INA E. ananas after 2 days. Because of the high selective potential of these media and the relatively simple epiphytic profile of tea gemmisphere, these two INA bacteria could exclusively be isolated, making the quantitative population analysis possible.
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