Abstract
Eukaryotic translation initiation factor 4E (eIF4E) plays a key role in the infection of potyviruses in susceptible plants by interacting with viral genome-linked protein (VPg). Sugarcane (Saccharum spp.) production is threatened by mosaic disease caused by Sugarcane mosaic virus (SCMV), Sorghum mosaic virus (SrMV), and Sugarcane streak mosaic virus (SCSMV). In this study, two eIF4Es and their isoform eIF(iso)4E and 4E-binding protein coding genes were cloned from sugarcane cultivar ROC22 and designated SceIF4Ea, SceIF4Eb, SceIF(iso)4E, and ScnCBP, respectively. Real-time quantitative PCR analysis showed different expression profiles of these four genes upon SCMV challenge. A subcellular localization assay showed that SceIF4Ea, SceIF4Eb, SceIF(iso)4E, and ScnCBP were distributed in the nucleus and cytoplasm. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays showed that SceIF4Ea/b and SceIF(iso)4E were selectively employed by different sugarcane mosaic pathogens, i.e., SCMV-VPg interacted with SceIF4Ea/b and SceIF(iso)4E, SrMV-VPg interacted with both SceIF4Eb and SceIF(iso)4E, and SCSMV-VPg interacted only with SceIF(iso)4E. Intriguingly, the BiFC assays, but not the Y2H assays, showed that ScnCBP interacted with the VPgs of SCMV, SrMV, and SCSMV. Competitive interaction assays showed that SCMV-VPg, SrMV-VPg, and SCMV-VPg did not compete with each other to interact with SceIF(iso)4E, and SceIF(iso)4E competed with SceIF4Eb to interact with SrMV-VPg but not SCMV-VPg. This study sheds light on the molecular mechanism of sugarcane mosaic pathogen infection of sugarcane plants and benefits sugarcane breeding against the sugarcane mosaic disease.
Highlights
IntroductionSugarcane mosaic disease is widespread in sugarcane-growing countries [4,5,6,7,8,9,10,11,12,13,14,15] and causes heavy yield losses [16,17]
Sugarcane (Saccharum sp.) is the most important sugar and energy crop worldwide [1,2,3].Sugarcane mosaic disease is widespread in sugarcane-growing countries [4,5,6,7,8,9,10,11,12,13,14,15] and causes heavy yield losses [16,17]
The main causal agents for the sugarcane mosaic disease are Sugarcane mosaic virus (SCMV), Sorghum mosaic virus (SrMV), and Sugarcane streak mosaic virus (SCSMV), which belong to the Potyviridae family, with SCMV and SrMV being members of the Potyvirus genus [20], whereas SCSMV is a member of Poacevirus genus [21,22,23]
Summary
Sugarcane mosaic disease is widespread in sugarcane-growing countries [4,5,6,7,8,9,10,11,12,13,14,15] and causes heavy yield losses [16,17]. In the 1920s, sugarcane mosaic disease almost collapsed the sugar industry in Argentina, Brazil, and Louisiana [18]. Sugarcane mosaic disease is controlled by planting resistant sugarcane cultivars, it continues to be a threat to the sugarcane industry [4,15,17,19].
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