Abstract
The selectivity of inhibition of herpesvirus deoxyribonucleic acid synthesis by acycloguanosine, 2'-fluoro-5-iodo-aracytosine, and (E)-5-(2-bromovinyl)-2'-deoxyuridine was determined by isopycnic banding of (32)P-labeled deoxyribonucleic acid from herpesvirus-infected and uninfected cells.
Highlights
The selectivity of inhibition of herpesvirus deoxyribonucleic acid synthesis by acycloguanosine, 2'-fluoro-5-iodo-aracytosine, and (E)-5-(2-bromovinyl)-2'-deoxyuridine was determined by isopycnic banding of 32P-labeled deoxyribonucleic acid from herpesvirus-infected and uninfected cells
The selective action of acycloguanosine (ACG), 2'-fluoro-5-iodoaracytosine (FIAC), and (E)-5-(2-bromovinyl)-2'-deoxyuridine (BrVDU) on viral and cellular deoxyribonucleic acid (DNA) synthesis in herpes simplex virus type 1 (HSV-1)-infected and uninfected Vero cells has been determined by isopycnic banding of DNA in CsCl gradients
No effect on DNA synthesis in uninfected cells could be detected at this concentration and 310,M ACG was required for a 50% inhibition
Summary
The test compounds were added to the medium 1 h postinoculation, and [32P]. Orthophosphate (5 to 10 ,uCi/ml) was added 3 h postinoculation. The cells were harvested after 16 h of incubation, and cellular and viral DNA were separated in CsCl gradients. [3H]thymidine-labeled DNA from infected and untreated cells was used as an internal density marker (not shown). The methods have been described previously (Larsson and Oberg, in press). 0.025 M ACG; (C) 1.0 1M ACG; (D) control; (E) 0.025 pM FIAC; (F) 0.5 pM FIAC; (G) control; (H) 0.1 pLM.
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