Abstract
A polysaccharide was purified from the slime of a myxomycete, Physarum polycephalum , and its inhibitory effect on eukaryotic DNA polymerases was examined. Almost all the calf thymus DNA polymerase α activity was inhibited with higher than 0.2 mg/ml of the polysaccharide, when the assay was carried out with activated DNA as a template. The inhibitory effect occurred regardless of the amounts of the enzyme and deoxyribonucleotides, however, kinetic analysis revealed that the inhibition occurs competitively with the template DNA, the K i value being 4 μg/ml. Inhibition was observed for DNA polymerase α, but not for DNA polymerases β and γ from various eukaryote species.
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