Abstract
The authors report on an aptamer-based method for the detection of S. Typhimurium. The aptamers were identified by using a modified cell-based SELEX method (cell-SELEX) by adopting an alternating negative-positive selection strategy. Each cross-reactive population was used separately, with a focus on generating strain-specific aptamers in order to enhance selectivity. The aptamers were characterized and chemically modified with thiol groups at the 5′ end which enables covalent binding to gold nanoparticles on a screen printed carbon electrode (GNP-SPCE). The aptamer-modified GNP-SPCE was subsequently applied to impedimetric sensing of S. Typhimurium in water and food samples. Response is linear in the 10 to 105 CFU⋅mL−1 concentration range, and the limit of detection is ~10 CFU⋅mL−1. The assay can distinguish S. Typhimurium from S. Typhi and has been validated in water and spiked egg samples.
Published Version
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