Abstract
The direct propagation of newly formed human hybridomas in serum-free medium selects for hybrids with metabolism best suited to growth in this environment. Under optimal culture conditions, this procedure results in the generation of antigen-specific human hybridomas comparable in frequency, stability, and antibody secretion rate to that obtained with murine hybridomas. After a transient phase of a few days in the appropriate selection medium supplemented with 1% serum, hybridomas grow in serum-free medium in stationary cultures with a cell doubling time of 15–25 h and an antibody production rate averaging 12 μg/10 6 cells/day. Clones propagated in bioreactors exhibited a cell doubling time of 29–35 h and an antibody secretion rate of 10–21 μg/10 6 cells/day.
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