Selective Filter Effect Induced by Cu(2+) Adsorption on the Fluorescence of a GdVO4:Eu Nanoprobe.

Abstract

Human blood contains substantial amounts of metal ions such as Mg(2+), Ca(2+), Fe(2+), Cu(2+), Zn(2+), Cd(2+), Pb(2+), and Al(3+). Most biomedical applications of nanoparticles require understanding the influence of these metal ions because adsorbed metal ions can affect the function of nanoparticles to limit their sensitivity, performance, stability, and/or resolution in applications. In the present work, the adsorption of various metal ions at the surface of GdVO4:Eu nanoparticles was studied to assess their spectral filter effect on the fluorescence of GdVO4:Eu. Due to the negative surface potential, the electrostatic attraction caused an intensive adsorption reaction of GdVO4:Eu nanoparticles with metal cations. Compared to the adsorption of other common metal ions in human blood, the distinct fluorescence quenching of GdVO4:Eu was induced in the presence of Cu(2+) ions. On the basis of the UV-vis absorption spectrum of an aqueous CuCl2 solution and reflectance spectrum of Cu(OH)2, in which the surroundings of Cu(2+) ions are supposedly similar to the hydroxylated surface of GdVO4:Eu nanoparticles, it is proposed that the complementary overlap of the emission band of GdVO4:Eu with the absorption band of Cu(2+) results in the effective filter effect to quench the red emission. Because GdVO4:Eu nanoparticles are attractive candidates for applications as magnetic/fluorescent multimodal nanoprobes, it is important to recognize that the average amount of Cu(2+) ion in human blood is sufficient to interfere with or limit the fluorescence probe function of GdVO4:Eu nanoparticles.