Selective Extraction of (−)Epigallocatechin Gallate from Green Tea Leaves Using Two-Stage Infusion Coupled with Membrane Separation

Abstract

A novel method for the extraction of (−)epigallocatechin gallate of high purity from green tea leaves is proposed in this study. The method comprised a two-stage water-based extraction followed by successive use of microfiltration and ultrafiltration. Microfiltration was used as a pretreatment to ultrafiltration. The best process conditions of each unit operation were estimated by performing well-planned experimentations. The clarified liquor was dried to powder by freeze drying. Chemical analyses revealed that the tea powder contained about 90% of polyphenols. The purity of (−)epigallocatechin gallate was found to be about 80%, while its average yield was 1.22 g/l. The method outlined in this study may have remarkable importance for the bulk production of high-purity (−)epigallocatechin gallate with potential application in pharmaceutical, cosmetic, and food-processing industries. Besides being a green process, this method can be easily scaled up for the commercial production of (−)epigallocatechin gallate.