Abstract
Embryogenic callus cultures of Ipomoea batatas Poir. produce fast growing non-embryogenic material which soon dominates the cultures. Our objective was to selectively enhance the proliferation of the embryogenic fraction. For this, the effect of BAP and 2,4-D concentrations on growth of embryogenic and non-embryogenic callus were studied and consequently, nutrient media for the production and indefinite maintenance of embryogenic callus without embryo formation were defined. Selective proliferation of embryogenic callus was obtained on solid media with 10 μM 2,4-D and 1 μM BAP and in liquid media with 5 μM 2,4-D. Selective proliferation of non-embryogenic callus occurred in liquid medium with 1 μM 2,4-D. In embryogenic liquid culture, embryos were produced with 0–2 μM 2,4-D. Increasing 2,4-D concentration from 0 to 2 μM in these cultures restricted embryo development.
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