Abstract
Abstract A simple determination method of histamine in urine by solvent extraction and atomic absorption spectrophotometry has been established. The method is based on the formation of histamine–Cu(II) chelate cation, followed by the extraction of ion-associates with tetrabromophenolphthalein ethyl ester into 1,2-dichloroethane. Histamine in urine can be successfully determined without separation and purification from other histamine derivatives and amines. A linearity is obtained in the concentration range (0.4–4)×10−5 M (1 M=1 mol dm−3) histamine in aqueous solutions. The relative deviation is ca. 1.8%.
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