Selective Determination of DNA by Its Enhancement Effect on the Fluorescence of the Eu3+-Tetracycline Complex

Abstract

Fluorescence enhancement of the Eu 3+ -tetracycline complin by DNA or RNA was studied. Studies involving natural and denatured fish sperm DNA, calf thymus DNA, and yeast RNA revealed that double-stranded and single-stranded DNA is capable of enhancing the fluorescence of the Eu 3+ -tetracgcline complin, the RNA showed very little enhancement effect, which founded a this for selective determination of DNA in the presence of RNA Maximum fluorescence was produced in the pH range8.0-9.7, with maximum incitation and emission wavelengths at 398 and 615 nm, respectively. Under optimal conditions, the calibration graphs were linear between 0.02 and 1.0 μg mL -1 for both double-stranded calf thymus and fish sperm DNA the corresponding detection lights were 0.01 μg mL -1 . The relative standard deviation (seven replicates) was within 3.0% in the linear rye. DNA could be determined in the presence of yeast RNA. If the time-resolved mode was used, the low end of the linear rye could be extended to 0.005 μg mL -1 , with a detection limit of 0.003 μg mL -1 . The mechanism for the fluorescence enhancement was also studied